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Cellulase »Onozuka« RS from Trichoderma viride ca. 2 U/mg
CAS [9012-54-8]
A multi-component enzyme system (1). Although the preparation has high cellulase activity (EC 3.2.1.4), it still contains hemicellulases, and it degrades mannans, xylans, galactomannans, pectins and other polysaccharides. Contains about three times as high xylanase activity as Cellulase Onozuka R-10 (cat. no. 16419). Widely used for the isolation of protoplasts, for its ability to degrade cell walls, often in combination with Macerozyme R-10 (cat. no. 28032) (2).
Temperature optimum: 50 - 60 °C
pH-optimum: pH 4 - 5
Unit definition: 1 U catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent (3).
Extraneous activities: Contains α-amylase, pectinase, protease and hemicellulase.
References:
- Beldman, G. et al. (1985) Eur. J. Biochem. 146, 301-8
- Potrykus, J. & Shillito, R.D. (1986) Methods Enzymol. 118, 549-78
- Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
- Lendl & Bauer (1989) Zell- und Gewebekultur, Gustav Fischer Verlag, 147ff.
XN
Harmful
R: 42
S: 22 - 24 - 36/37
EG-Index: 647-002-00-3 • EINECS: 232-734-4 • WGK: 1 • HS: 35079090
Storage Temperature: +2 °C to +8 °C
| Cat.No. | Size | EUR |  |
|---|---|---|---|
| 16420.01 | 1 g | inquire* | |
| 16420.02 | 5 g | inquire* | |
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Additional Information
Protoplast Preparation - Recipes
Cellulases: General Information
