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HomeEnzymologyEnzymes › Cellulase »Onozuka« R-10 from Trichoderma viride ca. 1 U/mg

Cellulase »Onozuka« R-10 from Trichoderma viride ca. 1 U/mg

CAS [9012-54-8]
 XN 
Harmful

R: 42
S: 2 - 22 - 24 - 36/37
EG-Index: 647-002-00-3 • • EINECS: 232-734-4WGK: 1HS: 35079090
Storage Temperature: +2 °C to +8 °C

A multi-component enzyme system (1). Although the preparation has high cellulase activity, it still contains hemicellulases, and it degrades mannans, xylans, galactomannans, pectins and other polysaccharides. Widely used for the isolation of protoplasts, for its ability to degrade cell walls, often in combination with Macerozyme R-10 (cat. no. 28032) (2).
Temperature optimum: 40 - 50 °C
pH-optimum: pH 4 - 5

Unit definition: 1 U catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent (3).
Extraneous activities: α-amylase ca. 0.8 U, pectinase ca. 0.4 U, protease ca. 0.01 DMC-U, hemicellulase ca. 1 U/mg (1 U catalyzes the liberation of 1 µmole reducing groups from xylan per hour at 37 °C, pH 5.5, calculated as xylose).

References:
  1. Beldman, G. et al. (1985) Eur. J. Biochem. 146, 301-8
  2. Potrykus, J. & Shillito, R.D. (1986) Methods Enzymol. 118, 549-78
  3. Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
  4. Lendl & Bauer (1989) Zell- und Gewebekultur, Gustav Fischer Verlag, 147ff.

Cat.No. Size EUR
16419.02 2.5 g inquire*  
16419.03 10 g inquire*  
16419.05 50 g inquire*  
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PDF Downloads 
Specifications
AnleitungSicherheitsdatenblatt
ManualMaterial Safety Data Sheet


Additional Information 
Protoplast Preparation - Recipes
Cellulases: General Information

Thursday, March 11, 2010



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R 42

Deutsch:

Sensibilisierung durch Einatmen möglich.

English:

May cause sensitisation by inhalation.

Français:

Peut entraîner une sensibilisation par inhalation.

Italiano:

Può provocare sensibilizzazione per inalazione.

Español:

Posibilidad de sensibilización por inhalación.

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S 2

Deutsch:

Darf nicht in die Hände von Kindern gelangen.

English:

Keep out of the reach of children.

Français:

Conserver hors de portée des enfants.

Italiano:

Conservare fuori della portata dei bambini.

Español:

Manténgase fuera del alcance de los niños.

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S 22

Deutsch:

Staub nicht einatmen.

English:

Do not breathe dust.

Français:

Ne pas respirer les poussières.

Italiano:

Non respirare le polveri.

Español:

No respirar el polvo.

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S 24

Deutsch:

Berührung mit der Haut vermeiden.

English:

Avoid contact with skin.

Français:

Éviter le contact avec la peau.

Italiano:

Evitare il contatto con la pelle.

Español:

Evítese el contacto con la piel.

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S 36/37

Deutsch:

Bei der Arbeit geeignete Schutzhandschuhe und Schutzkleidung tragen.

English:

Wear suitable protective clothing and gloves.

Français:

Porter un vètement de protection et des gants appropriés.

Italiano:

Usare indumenti protettivi e guanti adatti.

Español:

Úsense indumentaria y guantes de protección adecuados.

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