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Cellulase »Onozuka« R-10 from Trichoderma viride ca. 1 U/mg
CAS [9012-54-8]
XN
Harmful
R: 42
S: 2 - 22 - 24 - 36/37
EG-Index: 647-002-00-3 • • EINECS: 232-734-4 • WGK: 1 • HS: 35079090
Storage Temperature: +2 °C to +8 °C
A multi-component enzyme system (1). Although the preparation has high cellulase activity, it still contains hemicellulases, and it degrades mannans, xylans, galactomannans, pectins and other polysaccharides. Widely used for the isolation of protoplasts, for its ability to degrade cell walls, often in combination with Macerozyme R-10 (cat. no. 28032) (2).
Temperature optimum: 40 - 50 °C
pH-optimum: pH 4 - 5
Unit definition: 1 U catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent (3).
Extraneous activities: α-amylase ca. 0.8 U, pectinase ca. 0.4 U, protease ca. 0.01 DMC-U, hemicellulase ca. 1 U/mg (1 U catalyzes the liberation of 1 µmole reducing groups from xylan per hour at 37 °C, pH 5.5, calculated as xylose).
References:
- Beldman, G. et al. (1985) Eur. J. Biochem. 146, 301-8
- Potrykus, J. & Shillito, R.D. (1986) Methods Enzymol. 118, 549-78
- Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
- Lendl & Bauer (1989) Zell- und Gewebekultur, Gustav Fischer Verlag, 147ff.
| Cat.No. | Size | EUR |  |
|---|---|---|---|
| 16419.02 | 2.5 g | inquire* | |
| 16419.03 | 10 g | inquire* | |
| 16419.05 | 50 g | inquire* | |
| *Please log in to see your actual prices. | |||
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Additional Information
Protoplast Preparation - Recipes
Cellulases: General Information
