(Stem bromelain) EC 3.4.22.32 • Mr ca. 33 000 • CAS [37189-34-7]
Suitable for blood group serology (1)
and degradation of proteins.
Unit definition: 1 DMC-U catalyzes the cleavage of 1 µmole peptide bond
from dimethyl casein per minute at 25 °C, pH 7.0 expressed in terms of newly
formed terminal amino groups (determined with TNBS) (2). Activity in other units: 1200 GDU units/g (1 GDU unit yields the
equivalent of 1 mg amino nitrogen from gelatin in 20 min. at 45 °C, pH 4.5).
1200 GDU units/g, 2400 FIP units/g (4).
References:
Gray, M.P. (1959) J. Lab. Clin. Med. 54, 155-7
Lin, Y. et al. (1969) J. Biol. Chem. 244, 789-93
Murach, T. (1976) Methods Enzymol. Vol. 45, Acad. Press, New York, p. 475-85
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BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen.
Vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen.
English:
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Français:
EN CAS DE CONTACT AVEC LES YEUX: rincer avec précaution à l’eau pendant plusieurs minutes. Enlever les lentilles de contact si la victime en porte et si elles peuvent être facilement enlevées. Continuer à rincer
Italiano:
IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
Español:
EN CASO DE CONTACTO CON LOS OJOS: Aclarar cuidadosamente con agua durante varios minutos. Quitar las lentes de contacto, si lleva y resulta fácil. Seguir aclarando.