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SERVACEL® Cellulose Ion Exchangers

Cellulose ion exchangers have a very loose hydrophilic network (no pores) with well separated charged groups. Ionic macro(bio)molecules are adsorbed to only a few sites which can be desorbed selectively under extremely mild conditions with high recoveries. pH stability is excellent between 3 and 12. Storage of DEAE-cellulose in 2 M NaOH for 4 months at 50 °C (122 °F) does not change capacity for proteins. In order to provide greater stability and pressure resistance, the cellulose matrix can be produced in »regenerated« microcrystalline form. Here the particles are granular and the amorphous (fibrous) part of the cellulose fibers is removed. For additional stabilization the fibers are crosslinked.
SERVACEL^® CM 52 and DEAE 52 are microcrystalline and thus allow a more dense packing: a superior resolution can be obtained compared with fibrous material. In addition, they are supplied in pre-swollen form to facilitate handling: they are ready-to-use after equilibration with the selected buffer system. They do not require the time consuming "pre-cycling" procedure (washing with acid resp. alkali) which is necessary with cellulose ion exchangers supplied as dry powder.

CM Cellulose - Cation exchangers (acidic type):
Cellulose is derivatized with CarboxyMethylchloride. The resulting weakly acidic high capacity exchanger can be used for neutral and basic proteins, peptides, lipids. 

DEAE Cellulose - Anion exchangers (basic types):

DiEthylAminoEthyl-groups are covalently bound to the cellulose hydroxylic groups. This anion exchanger can be applied for the chromatography of proteins with neutral or
acidic isoelectric points (IP), which can easily be determined by isoelectric focusing electrophoresis (IEF). Serum components, enzymes, peptides, polysaccharides, RNA-types, viruses, lipids and ribosomes have been separated. DEAE cellulose is suitable for purification of nucleic acids.