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Competent Cells

High-efficiency competent DG1 E. coli cells are supplied in a 96-tube tray. This convenient format gives you the freedom to perform from 1 to 96 transformations at a time, without thawing more tubes of cells than needed. You can remove individual tubes by cutting cap strips. Thus, this format eliminates the reduced efficiencies that occur as a result of repeated freezing and thawing and makes possible high-throughput transformation. Racks feature 8 x 12 holes pattern for rapid processing using multi-channel pipettors. Alpha-numeric, non-reversing, rack and lid ensure positive sample ID.

DG1 E. coli cells are offered in the 24, 48 and 96 formats, electro- or chemically- competent, so you can pick the one that best suits the needs of your experiment. The competent E. coli bacteria are consistently high quality, with high efficiencies (>1x10⁸ cfu/µg DNA for chemically-competent and >1x10⁹ cfu/µg DNA for electro-competent). The competent bacteria come complete with SOC media. Each tube contains 50 µl of competent E. coli, which is sufficient for one high efficiency transformation.

DG1 is convenient for use in cloning, plasmid preparation and library construction. It is endonuclease and recombination minus. It allows lacZalpha complementation for blue/white screening.

Genotype: mcrA delta(mrr-hsdRMA-mcrBC, modification-, restriction-) ∅80lacZdeltaM15 deltalacX74 recA1 araD139 delta(ara-leu)7697 galU galK rpsL endA1 nupG

Freedom to use anywhere from 1 to 96 reactions

No wasted reactions

High efficiency

Electro-competent cells: >10

⁹ cfu/µg

Chemically-competent cells: >10

⁸ cfu/µg

• 24, 48 or 96 format




Competent Cells



• DG1 chemically-competent cells  24 reactions
• DG1 chemically-competent cells  48 reactions
• DG1 chemically-competent cells  96 reactions
• DG1 electro-competent cells  24 reactions
• DG1 electro-competent cells  48 reactions
• DG1 electro-competent cells  96 reactions