Generon Ltd., UK
Glutathione Superflow (SuperGlu) is designed for rapid one-step purification
of glutathione-S-transferase-tagged proteins from bacteria, yeast, insect and
mammalian cultures. Glutathione is covalently coupled to Superflow matrix that
provides a high chemical and physical stability and excellent flow rates.
SuperGlu is made in the particle size range of 60 - 160 µm and has a dynamic
binding capacity of 10 mg recombinant GST/ml resin. SuperGlu is stable with
all commonly used buffers and reagents including 0.1 M NaOH and organic
solvents. Removal of GST tag (a naturally occuring protein with a molecular
weight of 26 kDa) can be undertaken while bound to the column or in solution
after elution using typical site-specific proteases such as PreScission™
Protease, Factor Xa or Thrombin.
The SuperGlu Superflow is a premium and
affordable resin for highly reproducible preparative purification of
GST-tagged proteins and GST pull downs. Use it in batch mode, with gravitiy
flow or spin columns and with LC-driven pumps. The resin is pre-swollen and
de-fined. It is transported in 20 % ethanol and is stable for up to 3 years at
2 - 8 °C from date of manufacture, the expiry date is recorded clearly on the
label of the bottle. The bulk resin is supplied in 10 ml, 25 ml and 100 ml
quantities.
