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GEL-FIX™/NetFix™ Gel Support
GEL-FIX™ products are sturdy supports to be incorporated during casting of gels. GEL FIX™ is made from polyester film. Both surfaces of the film are chemically activated providing covalent binding to the gel layer. The gel will adhere to the GEL-FIX™ film not only during electrophoresis but in all subsequent staining and fixing procedures, Thus, it supports the gel layer maintaining exact shape and size (no shrinking, no swelling, no tearing). For documentation, the film-bound gel can be air-dried just as easy at room temperature to result in a transparent film, ideally suited to store the original gel in the laboratory notebook or to acquire it for electronic date processing. GEL-FIX™ for Covers is used to protect the surface of a cast gel from drying out but prevents the gel from adhesion.
GEL-FIX™ for Agarose: for casting of agarose gels
0.18 mm polyester film, activated on both sides to bind agarose gel layers covalently
GEL-FIX™ for Covers: for covering gel surfaces
0.075 mm
polyester film, non-binding, suitable for polyacrylamide and agarose gels
NetFix™
is an inert, reinforcing fabric which serves as an ideal support for gel
layers. If the agarose or poly- acrylamide gel is subject to blotting after
electrophoresis we recommend NetFix™ for Agarose resp. NetFix™ for PAG. The
polyester fabric is activated to bind agarose resp. polyacrylamide. If a thin
gel is to be removed after electrophoresis and transferred onto the membrane
for blotting transfer it can easily get damaged (torn) because it is so thin.
Usage of activated GEL-FIX™, however, to support the gel layer is an obstacle
to blotting transfer because it must be removed physically before blotting.
Here, NetFix™ is the ideal choice. The net is incorporated into the matrix
during the casting process but will not interfere with electrophoresis or
blotting. NetFix™ allows convenient, safe handling as the gel is reinforced
with the NetFix™ layer. The gel will stay in shape while moving it. The
Polyester Sheet, untreated, is used as backing to support horizontal gels
prepared with NetFix™.
GEL-FIX™/NetFix™ Gel Support
