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Acrylamide Media

In heteroduplex analysis of DNA, the conformational properties of the double stranded molecules are used to distinguish different base pairing (i.e. mutations), based on the differing mobilities. Single Stranded Conformational Polymorphism (SSCP) refers to the differing migration properties of single stranded molecules affected by variations in base sequence. 

Handling of SERDOGEL SSCP 2 x Concentrate

SERDOGEL SSCP represents the gel matrix only. Other components like buffer, APS, TEMED, urea (if required) must be added before preparing the gel (see protocol below). SERDOGEL SSCP has been aliquoted under an inert atmosphere. The shelf life of an unopened bottle is 12 months if stored at 4 °C. For preparing a gel, degas the solution (3 minutes) before adding APS and TEMED. Polymerization time is comparable with normal acrylamide/bis-solutions (about 1 hour). Gels can be cast down to a dilution of 0.5 X (which corresponds to a 1:4 dilution of the SERDOGEL SSCP 2x concentrate).Further components for gel preparation, e.g. urea, glycerol, can be added. All common detection methods are applicable, i.e. silver staining and chemiluminescence. Detection can be performed as well using SERDOGEL SSCP.