Glutathion Superflow

Glutathione Superflow (SuperGlu) is designed for rapid one-step purification of glutathione-S-transferase-tagged proteins from bacteria, yeast, insect and mammalian cultures. Glutathione is covalently coupled to Superflow matrix that provides a high chemical and physical stability and excellent flow rates. SuperGlu is made in the particle size range of 60 - 160 µm and has a dynamic binding capacity of 10 mg recombinant GST/ml resin. SuperGlu is stable with all commonly used buffers and reagents including 0.1 M NaOH and organic solvents. Removal of GST tag (a naturally occuring protein with a molecular weight of 26 kDa) can be undertaken while bound to the column or in solution after elution using typical site-specific proteases such as PreScission™ Protease, Factor Xa or Thrombin.
The SuperGlu Superflow is a premium and affordable resin for highly reproducible preparative purification of GST-tagged proteins and GST pull downs. Use it in batch mode, with gravitiy flow or spin columns and with LC-driven pumps. The resin is pre-swollen and de-fined. It is transported in 20 % ethanol and is stable for up to 3 years at 2 - 8 °C from date of manufacture, the expiry date is recorded clearly on the label of the bottle. The bulk resin is supplied in 10 ml, 25 ml and 100 ml quantities.

  • Optimized for high-performance purification of GST-tagged proteins
  • Rapid one-step purification
  • Negligible ligand leakage
  • Typical binding capacity of 10 mg/ml resin
  • High flow rate
  • High recovery of product


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    2023

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    SERVA Catalog
    2023

    In detail you will find the following new products in catalog 2022:

    New glycosidases such as Endo F3, Endo S and Sialidase
    Blotting kits to match the "Easy Peel" 2D HPE™ gels for blotting horizontal 2D gels
    New protein standards, e.g. SERVA Triple Color Protein Standard I    and the SERVA Fluo-610 Protein Standard I, pre-stained for direct fluorescence detection
    BlueVertical™ PRiME™ blot module and gel casting stand for SERVA's BV-104   electrophoresis  tank
    he albumin portfolio has been expanded with the "Albumin bovine Fraction V, protease- free, low IgG"
    Ready-to-use CL HRP Western Blotting Substrates SERVALight "PreMix"
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    Electrophoresis by SERVA - Catalog

    • Isoelectric Focusing

    • SDS PAGE

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    • Native Electrophoresis

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    • Nucleic Acid Electrophoresis

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