96-well format assay kit for protein quantification. The kit bases on the
precipitation of proteins as insoluble dye complexes with acidic, methanolic
amido black 10B solution (1, 2). After precipitation the protein-dye complexes
are spinned down. The pellet is washed and resolubilized. The thereby released
dye amount is measured at 620 nm. Linearity from 100 to 1750 µg/ml protein. In
triple measurement 23 samples can be analysed (plus BSA standard and blank). Note:
Ampholytes bind to the dye as well and therefore simulate too high protein
concentrations. Protein measurement of analytic solutions for 2DGE must be
done before ampholyte addition.
Precise, reproducible, reliable assay
data
Completed in 45 min.
No interference with detergents or
reducing agents
High-throughput format (96-well plate)
Linear
detection range from approx. 100 - 1750 µg/ml protein
The kit
contains 10x dye concentrate, elution solution, bovine serum albumin as
standard reference, 96-well plates.
For his work on a new selective method to isolate and analyze phospho- and glycoproteins Fabio Polato from the University of Innsbruck received the SERVA Young Scientists Award 2010 during the "17. Arbeitstagung Mikromethoden in der Proteinchemie" (21 to 23 June 2010, Martinsried, Germany). Congratulations!
(in front: Fabio Polato (Universität Innsbruck) and Barbara Müller (SERVA, Heidelberg), background left: Helmut E. Meyer (Ruhr-Universität Bochum), right: Roland Kellner (MERCK, Darmstadt)
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